Compartmentalized biosynthesis of mycophenolic acid
نویسندگان
چکیده
منابع مشابه
Molecular basis for mycophenolic acid biosynthesis in Penicillium brevicompactum.
Mycophenolic acid (MPA) is the active ingredient in the increasingly important immunosuppressive pharmaceuticals CellCept (Roche) and Myfortic (Novartis). Despite the long history of MPA, the molecular basis for its biosynthesis has remained enigmatic. Here we report the discovery of a polyketide synthase (PKS), MpaC, which we successfully characterized and identified as responsible for MPA pro...
متن کاملBiosynthesis of mycophenolic acid: purification and characterization of S-adenosyl-L-methionine: demethylmycophenolic acid O-methyltransferase.
The final step in the biosynthesis of mycophenolic acid involves the transfer of a methyl group from S-adenosylmethionine to demethylmycophenolic acid. The enzyme, S-adenosylmethionine:demethylmycophenolic acid O-methyltransferase, was isolated from Penicillium stoloniferum and purified 2,700-fold by ammonium sulfate fractionation and diethylaminoethyl-cellulose and Sephadex G-200 chromatograph...
متن کاملAntitumor activity of mycophenolic acid.
n our laboratory in 4CS mice. (3) Mouse ascites hepatoma, MH134, was converted into ascitic form by Sato et al.*\ from a C3H/HeN mouse hapatoma induced after treatment with carbon tetrachloride by Andervont et al.5) It has been maintained in C3H/He mice with distinct strain specificity in tumor take in our laboratory. (4) Mouse ascites sarcoma, SR-C3H/He Th62, was originally induced with Schmid...
متن کاملMycophenolic acid in silage.
We examined 233 silage samples and found that molds were present in 206 samples with counts between 1 x 10(3) and 8.9 x 10(7) (mean, 4.7 x 10(6)) CFU/g. Mycophenolic acid, a metabolite of Penicillium roqueforti, was detected by liquid chromatography-mass spectrometry in 74 (32%) of these samples at levels ranging from 20 to 35,000 (mean, 1,400) microg/kg. This compound has well-known immunosupp...
متن کاملInvolvement of a natural fusion of a cytochrome P450 and a hydrolase in mycophenolic acid biosynthesis.
Mycophenolic acid (MPA) is a fungal secondary metabolite and the active component in several immunosuppressive pharmaceuticals. The gene cluster coding for the MPA biosynthetic pathway has recently been discovered in Penicillium brevicompactum, demonstrating that the first step is catalyzed by MpaC, a polyketide synthase producing 5-methylorsellinic acid (5-MOA). However, the biochemical role o...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2019
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.1821932116